Tag Archives: protein modification

Fantastic Sortases and where to find them

Since my bachelor thesis I use cloning on a regular basis. Back then to transform yeast, later I needed to clone in order to perform a yeast-two-hybrid screen while now everything has shifted to the expression and modification of proteins. Cloning of plasmids is easy due to the long known and appreciated restriction enzymes. You flank your coding sequence with unique restriction sites and ligate … Continue reading Fantastic Sortases and where to find them »